教育与工作经历
1998.09-2002.06 武汉大学化学与分子科学学院，化学基地班，学士
2002.07-2007.11 武汉大学化学与分子科学学院，有机化学，博士，导师：周翔教授
2007.11-2010.11 武汉大学化学与分子科学学院，讲师
2010.11-2018.09 武汉大学化学与分子科学学院，副教授
2013.11-2016.11 美国芝加哥大学 访问学者，博士后，导师：何川 教授
2018.09- 至今 武汉大学化学与分子科学学院，教授
研究领域与兴趣
长期从事核酸化学生物学的研究。基于化学探针等化学技术，融合分子生物学、基因组学、细胞生物学等手段，开发核酸表观遗传修饰、核酸结构以及核酸/蛋白质相互作用的研究技术，并开展相应的功能研究，以及开拓在临床疾病诊断方面的应用。
成绩与荣誉
1. 2020年湖北省科学技术奖励自然科学一等奖 (核酸的分子识别和调控，第2完成人)
2. 2018年获批国家自然科学基金优秀青年基金资助
3. 2015年湖北省楚天学者计划楚天学子
4. 2010年湖北省科学技术奖励自然科学奖一等奖（以核酸为靶目标的小分子的设计、合成及其生物活性研究，第2完成人）
5. 获武汉大学第五届青年教师教学竞赛一等奖 (2010年)
6. 获英国皇家化学会RSC “Young Chemist Award” 参会项目资助 (2009年)
7. 已主持国家自然科学基金面上项目4项，青年基金1项，承担国家基金委重大研究计划“基因信息传递过程中非编码RNA的调控作用机制”集成项目课题1项
8. 发表SCI论文80多篇，其中以共同第一/通讯作者在 Nat. Chem. Biol., Cancer Cell, Nat. Commun., J. Am. Chem. Soc., Angew. Chem. Int. Ed.等刊物上发表论文30多篇
代表性论文
1.Ruitu Lyu, Tong Wu, Allen C. Zhu, Diana C. West-Szymanski, Xiaocheng Weng, Mengjie Chen, Chuan He*. KAS-seq: genome-wide sequencing of single-stranded DNA by N3-kethoxal-assisted labeling. Nature Protocols, 2021, accepted.
2.Yafen Wang, Shaoqing Han, Ruoxi Ran, Anling Li, Huanyu Liu, Mingjun Liu, Yongwei Duan, Xiong Zhang, Zhigang Zhao, Shihui Song, Xiaocheng Weng*, Songmei Liu*, Xiang Zhou*, A longitudinal sampling study of transcriptomic and epigenetic profiles in patients with thrombocytopenia syndrome. Nature Communications 2021, 12, 5629.
3.Wang, Y. F.; Zhang, X.; Han, S. Q.; Yang, W.; Chen, Z. G.; Wu, F.; Liu, J. Z.; Weng, X. C.*; Zhou, X.*, Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase. Acs Central Science 2021, 7 (6), 973-979. (封面文章)
4.Wang, Q.; Tan, K. Y.; Wang, H.; Shang, J. H.; Wan, Y. Q.; Liu, X. Q.; Weng, X. C.; Wang, F. A.*, Orthogonal Demethylase-Activated Deoxyribozyme for Intracellular Imaging and Gene Regulation. Journal of the American Chemical Society 2021, 143 (18), 6895-6904.
5. Wang, B. Y.; Zhang, X.; Wang, Y. F.; Chen, K.; Wang, F.; Weng, X. C.*; Zhou, X.*, One-pot fluorescent assay for sensitive detection of APOBEC3A activity. RSC Chemical Biology 2021, 2 (4), 1201-1205.
6.Wang, B. Y.; Chen, Y.; Zhang, X.; Jiang, Z. R.; Wang, Y. F.; Chen, K.; Wang, F.; Weng, X. C.*; Zhou, X.*, A far-red emissive two-photon fluorescent probe for quantification of uracil in genomic DNA. Chemical Communications 2021, 57 (22), 2784-2787.
7.Li, W.; Yang, H. M.; Wang, Y. F.; Weng, X. C.*; Wang, F.*, Highly sensitive detection of 6mA at single-base resolution based on A-C mismatch. Analyst 2021, 146 (14), 4450-4453.
8.Huang, J. G.; Zhao, R. Q.; Mo, J.; Wang, F.; Weng, X. C.*; Zhou, X. *, N-3-Kethoxal-Based Bioorthogonal Intracellular RNA Labeling. Chembiochem 2021, 22 (9), 1559-1562.
9.Zhang, Y. J.; Zhang, Q. Y.; Weng, X. C.; Du, Y. H.*; Zhou, X., NEase-based amplification for detection of miRNA, multiple miRNAs and circRNA. Analytica Chimica Acta 2021, 1145, 52-58.
10.Huang, J. G.; Zhao, R. Q.; Qin, S. S.; Yang, S. X.;Li, W.; Mo, J.; Wang, F.; Du, Y. H.; Weng, X. C.*; Zhou, X., 4-Thiouridine-Enhanced Peroxidase-Generated Biotinylation of RNA. Chembiochem 2021, 22, 212-216.
11.Mo, J.; Chen, Z. G.; Qin, S. S.; Li, S.; Liu, C. G.; Zhang, L.; Ran, R. X.; Kong, Y.; Wang, F.; Liu, S. M.; Zhou, Y.; Zhang, X. L.; Weng, X. C.*; Zhou, X.*, TRADES: Targeted RNA Demethylation by SunTag System. Advanced Science 2020, 7 (19), 202001402.
12.Chen, Yuqi; Wu, Fan; Chen, Zonggui; He, Zhiyong; Wei, Qi; Zeng, Weiwu; Chen, Kun; Xiao, Feng; Yuan, Yushu; Weng, Xiaocheng; Zhou, Yu; Zhou, Xiang*, Acrylonitrile-Mediated Nascent RNA Sequencing for Transcriptome-Wide Profiling of Cellular RNA Dynamics. Advanced Science 2020, 7 (8), 1900997.
13.Weng, X.; Gong, J.; Chen, Y.; Wu, T.; Wang, F.; Yang, S.; Yuan, Y.; Luo, G.; Chen, K.; Hu, L.; Ma, H.; Wang, P.; Zhang, Q. C.*; Zhou, X.*; He, C.*, Keth-seq for transcriptome-wide RNA structure mapping. Nature Chemical Biology 2020, 16 (5), 489-492.
14.Yang, S.; Wu, F.; Peng, S.; Wang, F.; Chen, Y.; Yuan, Y.; Weng, X.*; Zhou, X., A m6A sensing method by its impact on the stability of RNA double helix. Chemistry undefinedamp; Biodiversity 2020, https://doi.org/10.1002/cbdv.202000050.
15.Liu, Y. Q.; Zhang, X.; Liu, M.; Xu, F.; Zhang, Q. Y.; Zhang, Y. J.; Weng, X. C.; Liu, S. M.; Du, Y. H.*; Zhou, X.*, Direct detection of circRNA in real samples using reverse transcription-rolling circle amplification. Analytica Chimica Acta 2020, 1101, 169-175.
16.Zou, G. R.; Liu, C. X.; Fang, Z. T.; Jia, S. K.; Yang, W.; Zhang, X.; Chen, M. K.; Tian, T.; Weng, X. C.; Zhou, X.*, Photocaged probes for spatiotemporal imaging. Sensors and Actuators B-Chemical 2019, 288, 113-119.
17.Li, W.; Zeng, W. W.; Chen, Y.; Wang, F.; Wu, F.; Weng, X. C.*; Zhou, X.*, Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity. Analyst 2019, 144 (15), 4472-4476.
18.Li, W.; Wang, F.; Chen, Y.; Weng, X. C.*; Zhou, X., A sensitive and radiolabeling-free method for pseudouridine detection. Analytical Biochemistry 2019, 581.
19.Chen, Y.; Yang, S.; Peng, S.; Li, W.; Wu, F.; Yao, Q.; Wang, F.; Weng, X.*; Zhou, X.*, N1-methyladenosine detection with CRISPR-Cas13a/C2c2. Chemical Science 2019, 10, 2975-2979
20.Zhong, X.; Yu, J. Y.; Frazier, K.; Weng, X. C.; Li, Y.; Cham, C. M.; Dolan, K.; Zhu, X. R.; Hubert, N.; Tao, Y.; Lin, F. F.; Martinez-Guryn, K.; Huang, Y.; Wang, T.; Liu, J. Z.; He, C.*; Chang, E. B.*; Leone, V.*, Circadian Clock Regulation of Hepatic Lipid Metabolism by Modulation of m(6)A mRNA Methylation. Cell Reports 2018, 25 (7), 1816-+.
21.Yuan, Y. S.; Hong, T. T.; Chen, Y.; Wang, Y. F.; Qiu, X. P.; Zheng, F.; Weng, X. C.*; Zhou, X.*, Luminescence Sensing for Qualitative and Quantitative Detection of 5-Methylcytosine. Analytical Chemistry 2018, 90 (16), 10064-10068.
22.Wu, F.; Liu, W. T.; Yang, S. X.; Yao, Q.; Chen, Y.; Weng, X. C.*; Zhou, X.*, An aptamer-based ligation-triggered rolling circle amplification strategy for ATP detection and imaging in situ. Journal of Photochemistry and Photobiology a-Chemistry 2018, 355, 114-119.
23.Wang, Y. F.; Liu, C. X.; Zhang, X.; Yang, W.; Wu, F.; Zou, G. R.; Weng, X. C.*; Zhou, X.*, Gene specific-loci quantitative and single-base resolution analysis of 5-formylcytosine by compound-mediated polymerase chain reaction. Chemical Science 2018, 9 (15), 3723-3728.
24.Wang, Y. F.; Liu, C. X.; Wu, F.; Zhang, X.; Liu, S.; Chen, Z. G.; Zeng, W. W.; Yang, W.; Zhang, X. L.; Zhou, Y.; Weng, X. C.; Wu, Z. G.; Zhou, X.*, Highly Selective 5-Formyluracil Labeling and Genome-wide Mapping Using (2-Benzimidazolyl)Acetonitrile Probe. Iscience 2018, 9, 423-+.
25.Su, R.; Dong, L.; Li, C.; Nachtergaele, S.; Wunderlich, M.; Qing, Y.; Deng, X.; Wang, Y.; Weng, X.; Hu, C.; Yu, M.; Skibbe, J.; Dai, Q.; Zou, D.; Wu, T.; Yu, K.; Weng, H.; Huang, H.; Ferchen, K.; Qin, X.; Zhang, B.; Qi, J.; Sasaki, A. T.; Plas, D. R.; Bradner, J. E.; Wei, M.; Marcucci, G.; Jiang, X.; Mulloy, J. C.; Jin, J.*; He, C.*; Chen, J.*, R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m(6)A/MYC/CEBPA Signaling. Cell 2018, 172 (1-2), 90-+.
26.Luo, G. Z.; Hao, Z. Y.; Luo, L. Z.; Shen, M. R.; Sparvoli, D.; Zheng, Y. Q.; Zhang, Z. J.; Weng, X. C.; Chen, K.; Cui, Q.; Turkewitz, A. P.; He, C.*, N-6-methyldeoxyadenosine directs nucleosome positioning in Tetrahymena DNA. Genome Biology 2018, 19.
27.Hong, T. T.; Yuan, Y. S.; Chen, Z. G.; Xi, K.; Wang, T. L.; Xie, Y. L.; He, Z. Y.; Su, H. M.; Zhou, Y.; Tan, Z. J.; Weng, X. C.*; Zhou, X.*, Precise Antibody -Independent m6A Identification via 4SedTTP-Involved and FTO-Assisted Strategy at Single-Nucleotide Resolution. Journal of the American Chemical Society 2018, 140 (18), 5886-5889.
28.Liu, C.; Wang, Y.; Yang, W.; Wu, F.; Zeng, W.; Chen, Z.; Huang, J.; Zou, G.; Zhang, X.; Wang, S.; Weng, X.; Wu, Z.; Zhou, Y.; Zhou, X.*, Fluorogenic labeling and single-base resolution analysis of 5-formylcytosine in DNA. Chemical Science 2017, 8 (11), 7443-7447.
29.Li, Z.; Weng, H.; Su, R.; Weng, X.; Zuo, Z.; Li, C.; Huang, H.; Nachtergaele, S.; Dong, L.; Hu, C.; Qin, X.; Tang, L.; Wang, Y.; Hong, G.-M.; Huang, H.; Wang, X.; Chen, P.; Gurbuxani, S.; Arnovitz, S.; Li, Y.; Li, S.; Strong, J.; Neilly, M. B.; Larson, R. A.; Jiang, X.; Zhang, P.; Jin, J.*; He, C.*; Chen, J.*, FTO Plays an Oncogenic Role in Acute Myeloid Leukemia as a N-6-Methyladenosine RNA Demethylase. Cancer Cell 2017, 31 (1), 127-141.
